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Sex Determination in Long-eared Owl (Asio otus) by means of PCR DNA amplification
Pecharová, Šárka ; Řezníček, Jan (advisor) ; Hačecká, Kristýna (referee)
This thesis is focused on determining the sex of the Long-eared Owl (Asio otus). Bird feathers were used during determination that were taken from owl`s back while capturing and ringing. We have attempted to differentiate the sex of captured Long-eared Owl by DNA isolation from blood platelets in the bird quail and subsequent PCR reaction. This thesis also describes the PCR method, which is one of the most used methods in the todays laboratories of many different field of science. I also mention the individual components that are needed for the process of this reaction, its history and its individual steps. Part of my diploma thesis is also the characteristic of the tested order and possible gender division based on morphological features. On this basis, there is also mention of the sexual dimorphism of birds, their plumage and its subsequent coloring. At birds and also at other animals are used different techniques of molecular determination of sex, which I also refer to in my work. Keywords Long-eared Owl (Asio otus), sex, chromosomes, feathers, colour, PCR, DNA
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Monitoring of the Wintering Long-eared Owl (Asio otus) in the Czech Republic Territory
Moravcová, Alberta ; Řezníček, Jan (advisor) ; Hačecká, Kristýna (referee)
This diploma thesis deals with the monitoring of the Long-eared Owl (Asio otus) on the wintering grounds in Kladno Kročehlavy between 2015 and 2017. The monitoring is focused on spatial activity linked to food sources and food analysis. The research aimed at finding out whether the maximum distance of night overflight is within five kilometers from the night shelter. Another focus was on the discovery of the variability of the food supply in relation to the weather over the past 10 years and the comparison of the data with the results of the already published works. Several methods were used in the research. Method of observation, capture into impact ornithological nets, ringing, collection and analysis of pellets and modern method of GPS telemetry. Using mentioned methods, it was found that the maximum distance of overflights linked to food sources is not only within five kilometers from the night shelter. These distances may vary in length. The research managed to track the overflight less than 30 kilometers away. Further findings on the content of food supply and weather showed that there has been only slight variation in the occurrence of the main boar loot in the last ten years. KEYWORDS Long-eared Owl (Asio otus), frequency, telemetry, night shelter, migration, monitoring
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Sex Determination in Long-eared Owl (Asio otus) by means of PCR DNA amplification
Pecharová, Šárka ; Řezníček, Jan (advisor) ; Hačecká, Kristýna (referee)
This thesis is focused on determining the sex of the Long-eared Owl (Asio otus). Bird feathers were used during determination that were taken from owl`s back while capturing and ringing. We have attempted to differentiate the sex of captured Long-eared Owl by DNA isolation from blood platelets in the bird quail and subsequent PCR reaction. This thesis also describes the PCR method, which is one of the most used methods in the todays laboratories of many different field of science. I also mention the individual components that are needed for the process of this reaction, its history and its individual steps. Part of my diploma thesis is also the characteristic of the tested order and possible gender division based on morphological features. On this basis, there is also mention of the sexual dimorphism of birds, their plumage and its subsequent coloring. At birds and also at other animals are used different techniques of molecular determination of sex, which I also refer to in my work. Keywords Long-eared Owl (Asio otus), sex, chromosomes, feathers, colour, PCR, DNA
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Sex determination of phenotypically undifferentiated bird species focused on the Long-eared Owl (Asio otus)
Hlasivcová, Kristýna ; Řezníček, Jan (advisor) ; Ehler, Edvard (referee)
The aim of the present thesis is to give a comparision on sex determination of phenotypically undifferentiated birds species focused on the Long-eared Owl (Asio otus). Compared to other species of owls is relatively a few researches on the Long-eared Owl and researches on sex differences have not led to the quantified results yet. Attention is focused on characteristics and mechanisms of birds coloration. This work includes the methods and results of colour perception and interpretation in sexual dimorphism. It focuses primarily on the differences in color of certain parts of the bird, such as the alula, secondary remiges and lower part of the wing. For those pieces is further evaluating color differences in sex and proposes solutions that could help in further researches on sex determination of the Long-eared Owl.
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Ecological Factors and their Impact on a Diet of the Long -eared Owl (Asio otus)
Gaďůrková, Kateřina ; Řezníček, Jan (advisor) ; Andreska, Jan (referee)
My thesis deals with ecology and diet of the Long - eared Owl (Asio otus). The study area a wintering place is situated near the town of Kladno. 1) The hypothesis that a high snow cover has an influence on the Long-eared Owl diet has been confirmed. 3) The thesis deals with the determination methods of small vertebrate species in Long - eared Owl's diet. The work deals with postcranial skelet as well. There was compilled the method of the sex determination in Common vole (Microtus arvalis). The sex determination was based on the morphological differences of the pelvic bones. The results (sex ratio) had been statistically tested and as such found significant. 2) There were also analyzed the skeletal remnants of the pelvic bones in the pellets. The study of the pelvic bones was carry out in order to find expected seasonal changes in the sex ratio in a dominant prey Common vole (Microtus arvalis). That hypothesis was not confirmed. Keywords: Long-eared owl (Asio otus), diet, postcranial skeleton, pelvic bones
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